The 2-Minute Rule for 지방이식
The 2-Minute Rule for 지방이식
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Do you have a protocol or suggested antibodies to execute ICC staining on my totally differentiated ALI cultures?
Very best benefits are attained when limiting prolonged cell exposure to ambient temperature problems. Consider preserving unused cells in the humidified incubator with five% CO2 at 37°C when performing bigger experiments.
Each of those cells has a task to Engage in. Analysis on how they operate and their interactions with other subsets continues to reveal possibilities for novel therapeutic methods.
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Set the “unstained” tube apart. To the “viability dye” sample, centrifuge the tube at 300 x g
EasySep™ is a fast and straightforward immunomagnetic cell separation technology that enables you to isolate pretty much any cell types from various sample sources in as very little as eight minutes. This online video describes the EasySep™ technological know-how.
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Certainly, you’ll discover the methods to execute an ICC staining in your epithelial cells cultured in the ALI in this protocol. Here's a listing of antibodies which can be useful for the characterization of airway cultures:
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